Took the same set of samples and ran them with Agilent SYBR (catalog #600548) and Invitrogen SYBR (catalog 11762-100).
The results:
Agilent SYBR: Ave. Ct=15.57, SD=0.30
Invitrogen SYBR: Ave. Ct=18.23, SD=0.93
dCt=2.66, Fold=6.33, P=6.9e-09
In fairness to Invitrogen, the primers had been optimized in the past using the Agilent reagent and from the spec sheets for the Invitrogen product, they recommend different extension temperatures than the Agilent mix.
For my next test, will compare Invitrogen vs. Fermentas (catalog #K0251).
UPDATE:
Invitrogen SYBR: Ave. Ct=17.45, SD=0.35
Fermentas SYBR: Ave. Ct=14.56, SD=0.25
dCt=2.89, Fold=7.41, P=5.0e-11
Disclaimer: merely reporting what happened; individual results may vary; all reagents were received at promotional pricing discounts; this blog post does not constitute any endorsement.
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